长－47麻疹病毒非编码区和P、M基因序列及载体构建

摘要

本发明提供了麻疹病毒长春－47疫苗株的所有非编码区和P、M编码区序列及其结构，P、M编码区编码的蛋白质氨基酸序列及其结构以及其非编码区序列在构建该疫苗病毒载体中的用途和载体构建方法。这些序列数据和方法可应用于使用该疫苗株病毒构建表达载体用于重组多价疫苗或基因治疗，还可用于麻疹病毒感染的的分子生物学诊断及其试剂的研制。

主权项

1.麻疹病毒长-47疫苗株(Measles virus Changchun-47 attenuatedstrain)基因组所有非编码区序列，共包括7个不连续的区域，为6个结构基因编码区隔开，分别是基因组3’末端非编码区、N/P、P/M、M/F、F/H、H/L基因间非编码区和基因组5’末端非编码区，其特征在于：(1)麻疹病毒-47疫苗株(Measles virus ChangChun-47 attenuatedstrain)基组3’末端非编码区序列由107个核苷酸组成，位于全长基因组的第1-107位核苷酸。3’-5’方向RNA序列为：UGGUUUGUUUCAACCCAUUCCUAUCAAGUUAGUUACUAGUAGAAGAUCACGUGAAUCCUAAGUUCUAGGAUAAUAGUCCCUGUUCUCGUCCUAAUCCCUAUAGGCUC(2)麻疹病毒长-47疫苗株(Measles virus Changchun-47 attenuatedstrain)基因组N/P基因间非编码区(共121个核苷酸)，位于全长基因组的第1686-1806位核苷酸，3’-5’方向RNA序列为：CACGCUCUCCGGCUCCUGGUCUUGUUGUAGGCGGAUGGGAGGUAGUAACAAUAUUUUUUGAAUCCUUGGUCCAGGUGUGUCGGCGGUCGGGUAGUUGGUAGGUGAGGGUGCUAACCUCGGC(3)为麻疹病毒长-47疫苗株(Measles virus Changchun-47 attenuatedstrain)基因组P/M基因间非编码区(共107个核苷酸)，位于全长基因组的第3331-3437位核苷酸，3’-5’方向RNA序列为：GAUGUCGAGUUGAAUGGACGGUUGGGGUACGGUCAGCUGGGUUGAUCAUGUUGGAUUUAGGUAAUAUUUUUUGAAUCCUCGUUUCACUAACGGAGGGUUCAAGGUGU(4)为麻疹病毒长-47疫苗株(Measles virus Changchun-47 attenuatedstrain)基因组M/F基因间非编码区(共1012个核苷酸)，位于全长基因组的第4446-5457位核苷酸，3’-5’方向RNA序列为：UGGCAUCACGGGUCGUUACGGGCUUUUGCUGGGGGGAGUGUUACUGUCGGUCUUCCGGGCCUGUUUUUUUGGGGGAGGCUUUCUGAGGUGCCUGGUUCGCUCUCCGGUCGGUCGUCGGCUGCCGUUCGCGCUUGUGGUCCGCCGGGGUCGUGUCUUGUCGGGGCUGUGUUCCGGUGGUGGUCGGUGGGGUUAGACGUAGGAGGAGCACCCUGGGGGCUCCUGGUUGGGGGUUCCGACGGGGGCUAGGUUUGGUGGUUGGCGUAGGGGUGGUGGGGGCCCUUUCUUUGGGGGUCGUUGACCUUCCGGGGAGGGGGAGAGGGAGUUGUGUUCUUGAGGUGUUGGCUUGGCGUGUUCGCUGGCUCCACUGGGUUGGCGUCCGUAGGCUGAGGGAUCUGUCUAGGAGAGAGGGGCCGUUUGAUUUGUUUUGAAUCCCGGUUCCUUGUAUGUGUGGGUUGUCUUGGGUCUGGGGCCGGGUGCCGAGGCGCGGGGGUUGGGGGCUGUUGGUCUCCCUCGGGGGUUGGUUAGGGCGUCCGAGGGGGCCACGGGUGUCCGUCCCUGUGGUUGGGGGCUUGUCUGGGUCGUGGGUUGGUAGCUGUUAGGUUCUGCCCCCCCGGGGGGGUUUUUUUCCGGGGGUCCCCGGCUGUCGGUCGUGGCGCUCCUUCGGGUGGGUGGGGUGUGUGCUGGUGCCGUUGGUUUGGUCUUGGGUCUGGUGGGACCCGGUGGUCGAGGGUCUGAGCCGGUAGUGGGGCGUCUUUCCUUUCCGGUGUUGGGCGCGUGGGGUCGGGGCUAGGCCGCCCCUCGGUGGGUUGGGCUUGGUCGUGGGUUCUCGCUAGGGGCUUCCUGGGGGCUUGGCGUUUCCUGUAGUCAUGGGGUGUCGGAGAGGUUCAGGGGGCCAGAGGAGGAGAAGAGCUUCCCUGGUUCUCUAGUUAGGUGGUGUGGGCUGCUGUGAGUUGAGGGGUGGGGAUUUCCUCUGUGGCCCUUAGGGUCUUAGUUCUGAGUAGGUUACAGGUAG(5)为麻疹病毒长-47疫苗株(Measles virus Changchun-47 attenuatedstrain)基因组F/H基因间非编码区(共160个核苷酸)，位于全长基因组的第7111-7270位核苷酸，3’-5’方向RNA序列为：AGGAGAUGUUGAGAACUUUGUGUUUACAGGGUGUUCAGAGGAGAAGCAGUAGUUCGUUGGUGGCGUGGGUCGUAGUUCGGGUGGACUUUAAUAGAGGCCGAAGGGAGACCGGCUUGUUAUAGCCAUCAAUUAAUUUUGAAUCCCACGUUCUAGUAGGUGU(6)为麻疹病毒长-47疫苗株(Measles virus Changchun-47 attenuatedstrain)基因组H/L基因间非编码区(共109个核苷酸)，位于全长基因组的第9125-9233位核苷酸，3’-5’方向RNA序列为：CCGACGAUCACUUGGUUAGAGUACUACAGUGGGUCUGUAGUCCGUAUGGGUGAUCACACUUUAUCUGUAGUCUUAAUUCUUUUUGCGUCCCAGGUUCACCAAGGGGCAA(7)麻疹病毒长-47疫苗株(Measles virus Changchun-47 attenuatedstrain)基因组5’末端非编码区序列(共109个核苷酸)，位于全长基因组的第15786-15894位核苷酸，3’-5’方向RNA序列为：AACCAACUUGAGGCCUUGGGAUUAGGACGGGAUCCACCAAUCCGUAAUAAACGUUAUAUAAUUUCUUUUGAAACUUUUAUGCUUCAAAGAUAAGGGUCGAAACAGACCA(8)上述基因的结构和功能。