| 摘要 |
PROBLEM TO BE SOLVED: To provide a bioluminescent protein that can be used to detect and determine the following analytes in organelles or on the inner and outer surface of the cytomembrane in the living cells without incision or cleavage where the analytes are selected from biologically important substances, for example, microorganisms such as cells, bacteria, viruses and protozoa, and substrates, metabolites, intracellular and extracellular signals, enzymes, antigens and nucleic acids. SOLUTION: A bioluminescent protein comprising a bioluminescent protein portion as a donor linked to an energy transfer protein portion as an acceptor by a link portion where the link portion comprising an interaction site for a selected analyte is prepared and the reaction site of the protein is allowed to react with the analyte mutually to produce light such that the light is produced having a physical property of a first characteristic when the analyte is interacting with the interaction site, and is produced having a second different characteristic of the property, when the analyte is not reacting with the interaction site. |
