METHOD FOR SEPARATING CULTURED CELL BY USING THERMORESPONSIVE HIGH-MOLECULAR COMPOUND AND RELATED DEVICE AND CULTURE VESSEL

摘要

PROBLEM TO BE SOLVED: To provide a technique for surely and efficiently separating and collecting cells or cell clusters under a microscope. SOLUTION: The objective device for separating cultured cells includes a foil-like heater 2 for heating the bottom of a thin copper plate 1 placed with a culture vessel 8 in which cells are cultured in a thermoresponsive high- molecular compound as the medium, a hole 3 for small-range light transmission opened at the center common to both the thin plate 1 and the heater 2, and an air jet hole 5 via which cooling air is blown against the bottom of the culture vessel facing on the hole 3; wherein the thin plate 1 and the heater 2 are in the form of thin plate usable by being set on the stage of a microscope and the culture vessel has the flat bottom 0.1-0.2 mm thick.