| 摘要 |
PROBLEM TO BE SOLVED: To provide a substrate for PP2C exhibiting physiological conditions related to a specific treatment, and to discover a modulation factor capable of being used for treating apoptosis, especially neuron apoptosis. SOLUTION: A combination of protein phosphatase 2C as an enzyme and phosphor BAD as a substrate. Phospho-BAD, i.e., a protein, is dephosphorylated at a position of Ser<155> . Further, the combination can be used in an in vitro screening of a ligand which modulates protein phosphatase 2C. The screening comprises the following stages: the protein phosphatase 2C is incubated together with phosphor BAD as a substrate and a ligand of the assay; and the decrease in phosphor BAD, and/or the increase in phosphate and/or BAD are detected. As a result, a new drug for treating apoptosis can be discovered.
|
