HIGH THROUGHPUT METHOD FOR DISCOVERY OF GENE CLUSTERS

摘要

A method for identifying gene cluster is disclosed. The method may be used f or identifying gene dusters involved in the biosynthesis of natural products. A small insert library of DNA fragments of genomic DNA and a large insert library of DNA fragments of genomic DNA are prepared. Fragments in the small insert: library are sequenced and compared by homology comparison under computer control to a database containing genes, gene fragments or proteins known to be involved in the biosynthesis o f microbial natural products. Fragments having similar structure to genes, gene fragment s or proteins known to be involved in the biosynthesis of naturally occurring metabolites are used as probes to screen the large insert library of genomic DNA to detect gene clusters involved in the biosynthesis of microbial natural products.