Method for preparing cell culture system

摘要

Disclosed is a method for preparing a cell culture system, which comprises the step of simultaneously culturing two or more kinds of cell species (e.g., macrophages and vascular smooth muscle cells) which are involved in formation of a lesion of a mammalian disease (e.g., formation of an arteriosclerotic lesion) in one cell culture vessel under separation of said two or more kinds of cells by using, for example, a cell filter. A cell culture system is provided in which an environment of a lesion of a particular disease is reproduced.