发明名称 |
Cell line comprising the promoter of cyclooxygenase-2-(cox-2) and a witness gene, and use thereof in the search of cox-2 transcriptional induction selective inhibitors. |
摘要 |
The cell lines comprises a DNA construct that comprises all or part of a promoter sequence of the gene coding for cyclooxygenase 2 (cox-2) and a reporter gene, operatively joined to each other, such that said promoter sequence of the cox-2 gene controls the expression of said reporter gene in response to a suitable stimulus. The assay method comprises bringing said cell line into contact with the compound to be assayed and determining the existence of a signal indicative of the expression of activity due to the reporter gene. This method is suitable for the induction at a transcriptional level of cox-2 by suitable stimuli. <IMAGE> |
申请公布号 |
ZA200200059(B) |
申请公布日期 |
2003.04.03 |
申请号 |
ZA20020000059 |
申请日期 |
2002.01.03 |
申请人 |
LABORATORIOS DEL DR. ESTEVE, S.A. |
发明人 |
MANUEL FRESNO ESCUDERO;MIGUEL ANGEL INIGUEZ PENA |
分类号 |
G01N33/50;C12N5/10;C12N9/02;C12N15/09;C12Q1/02;C12Q1/68;G01N33/15 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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