发明名称 |
Novel isozyme of autoclavable superoxide dismutase (SOD), a process for the identification and extraction of the SOD and use of the said SOD in cosmetic, food, and pharmaceutical compositions |
摘要 |
The invention relates to a novel purified isozyme of an autoclavable superoxide dismutase extracted from the plant Potentilla astrisanguinea Lodd. Var. orgyrophylla, said isozyme having the following characteristics, O2-. scavenging activity remains same before and after autoclaving; scavenges O2-. from sub-zero temperature of -20° C. to high temperature of +80° C.; O2-. scavenging activity at 25° C. for 30 days without adding any stabilizing agent such as polyols or sugars; O2-. scavenging activity in the presence of saline (0.9% sodium chloride) to 61.8% of the control (without 0.9% sodium chloride), stable at 4° C. for at least 8 months; contamination free and infection free from any living micro- and/ or macro-organism after autoclaving; possesses temperature optima at 0° C.; possesses a molecular weight of 33 kD under non-denaturating conditions; possesses a molecular weight of 36 kD under denaturating conditions; has clear peaks in UV range at 268 and 275 nm; has an enzyme turnover number of 19.53x104% per nmol per min at 0° C.; and requires Cu/Zn as a co-factor, method for the preparation of the purified isozyme of autoclavable superoxide dismutase and formulations containing the said autoclavable superoxide dismutase.
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申请公布号 |
US2003064494(A1) |
申请公布日期 |
2003.04.03 |
申请号 |
US20020274053 |
申请日期 |
2002.10.21 |
申请人 |
COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH |
发明人 |
KUMAR SANJAY;SAHOO RASHMITA;AHUJA PARAMVIR SINGH |
分类号 |
A23G4/00;A23G4/12;A61K8/66;A61K38/00;A61Q19/00;C12N9/02;(IPC1-7):C12N9/02 |
主分类号 |
A23G4/00 |
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