发明名称 |
STEM CELLS OF THE ISLETS OF LANGERHANS AND THEIR USE IN TREATING DIABETES MELLITUS |
摘要 |
Methods and compositions are described for the treatment of type I insulin- dependent diabetes mellitus and other conditions using newly identified stem cells that are capable of differentiation into a variety of pancreatic islet cells, including insulin-producing beta cells, as well as hepatocytes. Nesti n and ABCG2 have been identified as molecular markers for pancreatic stem cell s, while cytokeratin-19 serves as a marker for a distinct class of islet ductal cells. Methods are described whereby nestin and/or ABCG2-positive stem cells can be isolated from pancreatic islets and cultured to obtain further stem cells or pseudo-islet like structures. Methods for ex vivo differentiation o f the pancreatic stem cells are disclosed. Methods are described whereby pancreatic stem cells can be isolated, expanded, and transplanted into a patient in need thereof, either allogeneically, isogeneically or xenogenically, to provide replacement for lost or damaged insulin-secreting cells or other cells.
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申请公布号 |
CA2461861(A1) |
申请公布日期 |
2003.04.03 |
申请号 |
CA20022461861 |
申请日期 |
2002.09.26 |
申请人 |
THE GENERAL HOSPITAL CORPORATION |
发明人 |
NOLAN, ANNA LOUISE;LEECH, COLIN A.;ABRAHAM, ELIZABETH J.;HABENER, JOEL F.;LECHNER, ANDREAS;THOMAS, MELISSA K.;VALLEJO, MARIO;ZULEWSKI, HENRYK |
分类号 |
C12N15/09;A01K67/027;A61K;A61K31/436;A61K31/7088;A61K35/12;A61K35/39;A61K38/00;A61K38/22;A61K45/00;A61K48/00;A61P3/10;C12N;C12N5/02;C12N5/074;C12Q1/00;G01N33/53;(IPC1-7):C12Q1/00 |
主分类号 |
C12N15/09 |
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