Combination vaccine comprising toxoid of dermonecrotic toxin produced by Bordetella bronchiseptica

摘要

The present invention aims at providing an improved toxoid of dermonecrotic toxin produced by Bordetella and a toxoid mixture comprising said improved toxoid of dermonecrotic toxin produced by Bordetella and a toxoid of dermonecrotic toxin produced by Pasteurella. Described is a method for partially purifying dermonecrotic toxin which comprises bringing a dermonecrotic toxin-containing solution into contact with a chromatographic gel sulfated by direct sulfation or a chromatographic gel to which a sulfated molecule is covalently bonded to thereby make the dermonecrotic toxin adsorbed on the gel, and eluting the adsorbed dermonecrotic toxin from the gel. There is provided a toxoid obtained from dermonecrotic toxin produced by Bordetella, said dermonecrotic toxin being prepared by the above method for partially purifying dermonecrotic toxin, and a toxoid mixture prepared by mixing said toxoid and a toxoid of dermonecrotic toxin produced by toxigenic Pasteurella, said toxoid mixture being safe and effective for preventing the atrophic rhinitis in pigs.