发明名称 |
Adenoviral helper vectors |
摘要 |
The present invention is directed to improved helper vectors and cell lines for the production of pseudoadenoviral (PAV) vectors containing substantially reduced levels of contaminating helper vector. The invention provides for helper vectors for the production of substantially helper vector-free PAV stocks comprising phag C31 recombinase recognition sequences which, depending upon their arrangement within the helper vector, can prevent helper vector packaging. The invention also provides for improved cell lines for the production of substantially helper vector-free PAV stocks comprising a stably introduced novel circular PAV genome into the cell.
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申请公布号 |
US6541245(B1) |
申请公布日期 |
2003.04.01 |
申请号 |
US20000718034 |
申请日期 |
2000.11.21 |
申请人 |
GENZYME CORPORATION |
发明人 |
ROMANCZUK HELEN;WADSWORTH SAMUEL C.;BERTHELETTE PATRICIA |
分类号 |
C12N5/10;C12N15/06;C12N15/861;(IPC1-7):C12N15/73;C12N15/79;C12N15/66;C12N15/865 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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