发明名称 |
Method of removing nucleic acid contamination in amplification reactions |
摘要 |
The present invention provides a method of removing nucleic acid contamination in an amplification reaction which comprises use of a thermolabile DNase and a method of preventing or reducing false positive results due to carry-over in a nucleic acid amplification reaction, said method comprising using a thermolabile DNase to degrade carried-over non-target double-stranded DNA present in the amplification reaction mixture. A thermolabile DNase from the shrimp Pandalus borealis has been identified which is suitable for use in the methods of the invention.
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申请公布号 |
US6541204(B2) |
申请公布日期 |
2003.04.01 |
申请号 |
US20000485110 |
申请日期 |
2000.04.10 |
申请人 |
NORWEGIAN INSTITUTE OF FISHERIES & AQUACULTURE LTD. |
发明人 |
NILSEN INGE WALLER;SANDSDALEN ERLING;STENBERG EVEN |
分类号 |
C12N15/09;C08F2/00;C12N9/22;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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