Method for purifying haemoglobin by eluting the haemoglobin with tris(hydroxymethyl)aminomethane acetate buffer solution in an anion exchange chromatography column

摘要

A method for producing a purified hemoglobin product comprises: a) loading a hemoglobin solution onto an anion exchange chromatography column and b) injecting at least one tris(hydroxymethyl) aminomethane acetate buffer solution into the column with the buffer solution having a pH lower than that of the column; whereby a purified hemoglobin product elutes from the column. In step a) of the above method the loaded column can be initially equilibrated at a pH of greater than 8.7. In step b) of the above method the buffer solution can have a pH lower than 8.2. The optional step of injecting into the column of step a) at least eleven column void volumes of equilibrating buffer solution with a pH of 8.2 to 8.6 to thereby form a stepped pH gradient to remove contaminants can be added to the above method.