METHODS AND COMPOSITIONS FOR DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISMS

摘要

Methods and compositions for detecting a single nucleotide, in particular, a single nucleotide polymorphism (SNP) in a target nucleic acid molecule using a gap-sensitive flap endonuclease enzyme are disclosed. The methods involve annealing upstream and downstream probes to a target nucleic acid molecule such that an overlapping, non-complementary portion of the downstream probe forms a closed flap structure at the SNP site if it is present. If the SNP is not present an open flap structure forms with a gap at the SNP site. The closed flap structure is cleaved by a gap-sensitive flap endonuclease enzyme indicating the SNP is present in the target nucleic acid.