RECOMBINANT PLASMID DNA PHBS PROVIDING BIOSYNTHESIS OF SURFACE ANTIGEN OF HEPATITIS B VIRUS, METHOD OF CONSTRUCTION OF PLASMID, YEAST STRAIN PICHIA PASTORIS PS103 (PHBS) AS PRODUCER OF INDICATED ANTIGEN

摘要

FIELD: biotechnology, genetic engineering, microbiological and medicinal industry. SUBSTANCE: invention relates to constructed in vitro recombinant plasmid that provides synthesis of HBsAg. Yeast strain-producer of surface antigen of hepatitis B virus (HBsAg) containing the constructed in vitro plasmid provides synthesis of HBsAg. The prepared yeast strain Pichia pastoris (pHBS) is producer of surface antigen of hepatitis B virus (HBsAg) is deposited in All-Russian collection of industrial microorganisms at number VKPM Y-2532. Surface antigen of hepatitis B virus produced by plasmid (HBsAg) in strain Pichia pastoris PS103 is useful for preparing vaccine against hepatitis B. EFFECT: improved method of plasmid construction, valuable properties of strain. 3 cl, 2 dwg, 2 ex