| 摘要 |
The present invention provides compositions and improved methods for multi- site directed mutagenesis and DNA shuffling. The present compositions and methods provide increased mutation frequency and increase number of transformants which allow one to sequence only a few clones in order to identify the correct mutants and to obtain the desired mutant by screening large number of transformants in a short time. Moreover, the inclusion of FE N- 1,, PEF and optimized buffer and cycling conditions provided in the present invention should also facilitate random mutagenized library construction and the mutagenesis of large or difficult templates.
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