ASPARAGINE HYDROXYLATION OF THE CAD DOMAIN OF A HIF PROTEIN
摘要
A target asparagine residue of HIF 1 alpha and 2 alpha is hydroxylated at high oxygen tension to render HIF as a weak transcription factor. An asparagine hydroxylase hydroxylation motif and binding motif is proposed. A method of screening for agonists or antagonists of an asparagine hydroxylase is also proposed and involves mixing peptides or proteins having the hydroxylation and or the binding motif with asparagine hydroxylase and a candidate agonist or antagonist. The extent of inhibition or enhancement of binding; level of asparagine hydroxylation or transactivation may be measured depending on the nature of the protein or peptide. Additionally altered proteins resistant to hydroxylation are described as are nucleic acids encoding such proteins.
申请公布号
WO03025013(A1)
申请公布日期
2003.03.27
申请号
WO2002AU01290
申请日期
2002.09.18
申请人
ADELAIDE RESEARCH & INNOVATION PTY LTD;WHITELAW, MURRAY, L.;LANDO, DAVID;PEET, DANIEL, J.;GORMAN, JEFFREY, J.;LINKE, SARAH
发明人
WHITELAW, MURRAY, L.;LANDO, DAVID;PEET, DANIEL, J.;GORMAN, JEFFREY, J.;LINKE, SARAH