| 摘要 |
<p>Specific binding assay for an analyte in a sample uses: (a) a first receptor (R1) that has binding affinity for the analyte and is immobilised on a first solid phase; (b) a second receptor (R2) that has binding affinity for the analyte, and (c) a binding factor that has binding affinity both for the analyte and for a third receptor (R3) that is immobilised on the first solid phase or on a second solid phase, where the binding factor has no binding affinity for R1 and is not labelled. The assay comprises contacting the sample with R1 and R2, contacting the resulting immunocomplexes with the binding factor and R3, and determining the amount of analyte bound to the first solid phase or, if present, the second solid phase.</p> |
