Method for identifying or isolating a molecule and molecules identified thereby

摘要

The invention describes methods for identifying a molecule of interest, as well as nucleic acid molecules which encode it, and binding partners for it. A cDNA library from a cell expressing the target is prepared and expressed in host cells. Lysates of the host cells are screened with a sample and treated to remove interfering binding partners. The treatment involves contact of the sample to lysates of transfected host cells and host cells transfected or transformed with the same vector used to make the cDNA library. Also part of the invention are antigens and cDNA identified using the methodology.