发明名称 |
INDUCIBLE ALPHAVIRAL GENE EXPRESSION SYSTEM |
摘要 |
The present invention provides novel expression vectors which permit tight regulation of gene expression in eucaryotic cells. More specifically, the invention provides DNA vectors comprising nucleotide sequences that are transcribed to form RNA molecules which are then replicated by a temperature-sensitive replicase to form additional RNA molecules. The RNA molecules produced by replication contain a nucleotide sequence which may be translated to produce a protein of interest or which encode one or more untranslated RNA molecules. Also provided are methods for producing heterologous proteins and untranslated RNA molecules. Further provided are methods for administering heterologous proteins and untranslated RNA molecules to individuals. In addition, pharmaceutical compositions are provided comprising the DNA and RNA molecules of the invention and a pharmaceutically acceptable carrier.
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申请公布号 |
US2003053988(A1) |
申请公布日期 |
2003.03.20 |
申请号 |
US19990275883 |
申请日期 |
1999.03.25 |
申请人 |
STERNE, KESSLER, GOLDSTEIN & FOX PLLC |
发明人 |
RENNER WOLFGANG A.;NIEBA LARS;BOORSMA MARCO |
分类号 |
A01K67/027;A61K48/00;C07K14/18;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N7/04;C12N9/12;C12N15/09;C12N15/86;(IPC1-7):A61K48/00;C12N7/00 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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