发明名称 |
SEQUENCE-DEPENDENT GENE SORTING TECHNIQUES |
摘要 |
This invention provides a method of sorting genes comprising: (1) preparing ds cDNA molecules from mRNA molecules; (2) digesting the ds cDNA molecules; (3) ligating to the digested cDNA molecules a set of dsDNA oligonucleotide adaptors; (4) amplifying the ligated cDNA molecules; and (5) sorting the amplified cDNA molecules into non-redundant groups. This invention also provides two additional methods of sorting genes. This invention further provides a method of making sub-libraries of ligation sets and a method of making sub-libraries of genetic vectors.
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申请公布号 |
WO0175180(A3) |
申请公布日期 |
2003.03.20 |
申请号 |
WO2001US09392 |
申请日期 |
2001.03.23 |
申请人 |
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发明人 |
ULANOVSKY, LEVY;MUGASIMANGALAM, RAJA;EINAT, PAZ;ZEZIN-SONKIN, DINA;SHLOMIT, GILAD |
分类号 |
C12N15/10;C12N15/66;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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