<p>The present invention provides a biosensor surface comprising a metal surface or metal membrane treated with a linker compound capable of binding to a physiologically active substance, a measurement chip for a biosensor having a physiologically active substance immobilized on the biosensor surface, a method for producing the same, and a method for measurement using the same. The present invention can provide a method for immobilizing a physiologically active substance on a metal surface in simple processes with high reliability while most of the molecules maintain activity and do not become detached.</p>