PREPARATION OF D-AMINO ACIDS BY DIRECT FERMENTATIVE MEANS

摘要

The present invention relates to materials and methods for production of natural and unnatural D-amino acids. In particular, the present invention relates to a fermentation method for the production of D-amino acids using recombinant host cells. Specifically, the invention relates to a method for producing a D-amino acid in a cell, comprising: (a) incorporating into the cell a D-aminotransferase gene and a L-aminodeaminase gene; (b) culturing the cell in a cell culture medium; and (c) isolating the D-amino acid from the cell culture medium. The invention also relates to a method for producing D-phenylalanine in a cell, comprising: (a) incorporating into the cell a D-aminotransferase gene, a L-aminodeaminase gene and means for increasing production of phenylpyruvate; (b) culturing the cell in a cell culture medium; and (c) isolating the D-phenylalanine from the cell culture medium. The invention also relates to the preparation of recombinant cells for use in the production of enantiomerically pure D-amino acids.