CELL-FREE SYNTHESIS AND ISOLATION OF NOVEL GENES AND POLYPEPTIDES

摘要

A method for the cell-free synthesis and isolation of novel genes and polypeptides is provided. Within one embodiment, an expression unit is constructed onto which semi-random nucleotide sequences a re attached. The semi-random nucleotide se- quences are first transcribed to produce RNA, and then translated under conditions such that polysomes are produced. Poly- somes which bind to a substance of interest are then isolated and disrupted; and the released mRNA is recovered. The mRNA is used to construct cDNA which is expressed to produce novel polypeptides.