摘要 |
Methods are provided for blocking non-specific and specific hybridization of nucleic acid samples on a microarray. The method of the present invention comprises applying a blocking reagent to the microarray, wherein the blocking reagent comprises modified nucleotide bases, preferably LNA (Locked Nucleic Acid -modified bicyclic monomeric units with a 2'-0- 4'-C methylene bridge). In further embodiments, the method includes applying a mixture including: a) a cDNA reagent obtained from mRNA of a target sample, the cDNA having a capture sequence; b) a dendrimer with a label for emitting a detectable signal and a second nucleotide sequence complementary to the capture sequence; and c) an blocking reagent containing LNA to a microarray, for producing a detectable signal from said label whereby a hybridization pattern is generated on the microarray. |
申请人 |
DATASCOPE INVESTMENT CORP.;KADUSHIN, JAMES, M.;GETTS, ROBERT, C.;ZAK, ARIEH, S. |
发明人 |
KADUSHIN, JAMES, M.;GETTS, ROBERT, C.;ZAK, ARIEH, S. |