NON-LINEAR AMPLITUDE DIELECTROPHORESIS WAVEFORM FOR CELL FUSION

摘要

An object of the invention is to provide a method of treating biological cel ls prior to subjecting the biological cells to cell fusion pulses which include s the step of treating the biological cells with pre-fusion electric field waveforms which change amplitude in a non-linear way with respect to time, such that the biological cells are first aligned with a relatively low amplitude, long duration pre-fusion electric field waveform and then compressed with a relatively high amplitude, short duration pre-fusion electric field waveform resulting in increased cell membrane contact prior t o being subjected to cell fusion. The non-linear pre-fusion electric field waveforms can change in a stepped way, in a continuous way, in a sigmoidal way, with step-wise increasing waveforms in adjacent steps, with step-wise increasing waveforms in non-adjacent steps, and in accordance with non-linea r algorithms.