| 摘要 |
The sensitivity, and therefore specificity, of the polymerase chain reaction is compromised by primer-dimer formation early in the amplification process. Described herein is a simple and novel technique to avoid the formation of primer-dimers. A target nucleic acid is first amplified in a "pre-amplification" reaction, wherein an extremely low concentration of primers bind to the target nucleic acid and not to each other. This allows for the efficient use of the DNA polymerase, deoxynucleoside triphosphates and other reaction components, to extend and amplify the target nucleic acid. |
