ENZYME OVER-PRODUCING TRANSGENIC MICROORGANISMS

摘要

A strain of Trichoderma reesei transformed to over-produce alpha -galactosidase. PCR primers are designed and used to link the alpha -galactosidase gene to the cellobiohydrolase promoter and terminator so that the alpha -galactosidase is expressed and expelled from the organism. Fermentation of the transformed organism produces alpha -galactosidase in the supernatant that is at a level of a least 20 IU per ml. Use of soybean hulls as an inducer increases the amount of alpha -galactosidase produced. The supernatant is filtered and concentrated to produce a liquid product that may be added to feed and food to improve conversion. The liquid concentrate may be dried, for example by spray drying, to produce a dried alpha -galactosidase product with an extended shelf life.