METHOD AND DEVICE FOR THE SYNTHESIS AND THE ANALYSIS OF SUPPORT-BOUND ARRAYS OF OLIGOMERS, ESPECIALLY OF PRIMER PAIRS FOR PCR, AS WELL AS OLIGOMER-CARRYING SUPPORTS

摘要

For synthesis of a support-bound array of oligomers with free ends A, whereby a temporary protecting group is provided on the 5'-OH end of the oligomers and permanent protecting groups on reactive side groups, it is proposed that the temporary protecting group is removed from the end B after combinatorial synthesis of the oligomers onto the support, the free ends B are then cross-linked even before splitting of the permanent protecting groups, then the covalent bond of the synthesised oligomers can be removed via the end A onto the support, resulting in free ends A, and a portion of the synthesised oligomers binds covalently to the support also after removal of the ends A from the support.