发明名称 METHOD AND APPARATUS FOR MEASURING FLUORESCENCE
摘要 A sample S is irradiated with pulsed pumping light supplied from a pumping light source 1, fluorescence generated by the sample S is detected by a photodetector 5 by way of a condensing optical system 3 and a spectroscope 4, and the fluorescence time waveform is subjected to a data analysis in a data processing unit 62 in a controller 6, so as to compute waveform data and physical quantities such as fluorescence lifetime. Here, the pumping light time waveform is fixedly arranged with respect to a time axis used for the data analysis, a plurality of fitting calculations are carried out while moving the fluorescence time waveform and fitting range from an initial position earlier than a pumping light peak to a later end position, and optimal measurement waveform data is selected according to a predetermined selection criterion. This realizes fluorescence measuring method and apparatus which can compute wave form data and the like accurately and efficiently regardless of fluctuations in the fluorescence time waveform, and a sample evaluating apparatus using the same. <IMAGE>
申请公布号 EP1291643(A1) 申请公布日期 2003.03.12
申请号 EP20010936885 申请日期 2001.06.07
申请人 HAMAMATSU PHOTONICS K.K. 发明人 WATANABE, MOTOYUKI;IGUCHI, KAZUYA
分类号 G01N21/64;(IPC1-7):G01N21/64;G01J3/44 主分类号 G01N21/64
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