发明名称 |
METHOD FOR PREPARING A FREEZE-DRIED COMPOSITION CONTAINING LACTIC ACID BACTERIA WITH ENHANCED BACTERIAL VIABILITY AND ACTIVITY DURING STORAGE AT ROOM TEMPERATURE AND RESULTING COMPOSITION |
摘要 |
The invention concerns a method for preparing a freeze-dried composition comprising the following steps: a) preparing a concentrate of lactic acid bacteria in a liquid medium, said liquid medium comprising: (i) a water-soluble antioxidant compound or a combination of water-soluble antioxidant compounds, and (ii) a compound or a combination of compounds for increasing the glass transition temperature of the freeze-dried product; b) freeze-drying the lipoprotected lactic acid bacteria prepared at step a), in accordance with the following freeze-drying steps: b1) freezing the concentrate of bacteria, b2) primary drying, preferably in mild operating conditions, b3) secondary drying until obtaining a freeze-dried composition having a water activity of about 0.1 and whereof the glass transition temperature (Tg) is higher by at least 20 DEG C than the predetermined storage temperature (Ts). The invention is applicable to biological and food industries. |
申请公布号 |
WO03018778(A2) |
申请公布日期 |
2003.03.06 |
申请号 |
WO2002FR02983 |
申请日期 |
2002.08.30 |
申请人 |
INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA);BEAL, CATHERINE;MARIN, MICHELE;CORRIEU, GEORGES;FONSECA, FERNANDA |
发明人 |
BEAL, CATHERINE;MARIN, MICHELE;CORRIEU, GEORGES;FONSECA, FERNANDA |
分类号 |
A23L29/00;C12N1/04;C12N1/20 |
主分类号 |
A23L29/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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