Fat reforming method with use of activated enzyme and activated enzyme deactivating method

摘要

A fat reforming method using an enzyme which has been activated by adding the enzyme to a system containing water and fat phases, activating the enzyme by a function of a boundary surface/layer between the water and fat phases, and removing the water and fat phases through freeze drying. Tetradecane may be used as the fat phase. Just after adding tetradecane into a water phase in which lipase is dissolved, though existing in water phase as shown in FIG. 4(a), the lipase moves towards the boundary surface since it has a tendency to gather around the boundary surface between the water and fat phases. The lipase moved to the boundary surface has hydrophobic portion at the side of the fat phase and a hydrophilic portion of the lipase at the side of the water phase, as shown in FIG. 4(b), and at the same time the lid covering the activating portion is opened by the function of the boundary surface. This condition is maintained even after the system is freeze-dried to remove the water and fat phases and the activated enzyme may be subsequently used to reform fat, and may be deactivated.