METHOD FOR PURIFYING RECOMBINANT PROTEINS EXPRESSED AS INSOLUBLE AGGREGATES

摘要

The invention relates to a method for solubilizing and purifying recombinant proteins, which are expressed in bacterial host cells and deposited as insoluble aggregates (inclusion bodies). The purification is based on the conversion of the inclusion bodies into soluble forms while using organic denaturation reagents and on the use of chromatographic methods. To this end , inorganic, alkaline mobile solvents that contain salt are selected, which, after a purification, make it possible to provide the recombinant proteins after neutralization in a physiologically acceptable form, which can be directly employed for medical use. The method is particularly suited for purifying allergens and allergen fragments.