METHOD OF JUDGING MISMATCH BETWEEN SINGLE-STRANDED NUCLEIC ACID MOLECULES

摘要

A method of judging whether or not a mismatch occurs between the sequences of a single−stranded nucleic acid molecule employed as a sample and another single−stranded nucleic acid molecule employed as a standard which comprises allowing a double−stranded nucleic acid molecule consisting of the standard single−stranded nucleic acid molecule with its complementary strand to coexist with the sample single−stranded nucleic acid molecule in the presence of a cationic polymer, measuring the ratio of the substitution of the sample single−stranded nucleic acid molecule by the complementary strand of the standard single−stranded nucleic acid molecule, and then judging the occurrence of the mismatch based on the measurement data. Using this method, the occurrence of a mismatch between single−stranded nucleic acid molecules can be accurately judged even in case where the mismatch occurs only in a single base.