METHOD AND DEVICE FOR INTEGRATED PROTEIN EXPRESSION, PURIFICATION AND DETECTION

摘要

A method for presenting a target protein or an array of target proteins for analysis is disclosed. The method includes the steps of forming a coding sequence; placing the coding sequence and protein-synthesis components capab le of expressing the target protein under selected protein-synthesis conditions in a well on a substrate, the well having a surface which has been functionalized with the second coil-forming peptide; expressing said coding sequence under such conditions, wherein the target protein so synthesized binds to the well through coil-coil heterodimer formation, and is thus presented for analysis in the well in captured form; and washing said well t o remove unbound components. The coding sequence includes a first nucleic acid sequence which encodes a first coil-forming peptide having a selected charge and being capable of interacting with a second, oppositely charged coil- forming peptide to form a stable .alpha.-helical coiled-coil heterodimer; an d a second nucleic acid sequence encoding the target protein. Also disclosed a re kits for use in practicing the method.