发明名称 |
Method for optical excitation of fluorophore marked DNA and RNA |
摘要 |
A method for optical excitation of fluorophore-labeled DNA and fluorophore-labeled RNA, particularly of specific localizations of DNA and RNA labeled by fluorescence in situ hybridization (FISH). It is the object of the method to make possible in a simple manner a high-contrast simultaneous excitation of a plurality of FISH fluorophores which have different fluorescence characteristics and are to be detected and displayed three-dimensionally. The excitation and detection of fluorophores at a depth of the biological material greater than 100 micrometers must be ensured. The FISH fluorophores and DNA markers are excited to fluorescence by a multiphoton excitation simultaneously by pulsed and non-pulsed radiation at a single wavelength in the range between 700 nm to 1000 nm, preferably between 760 nm and 820 nm. A total of 20 commercially available FISH fluorophores and DNA markers were tested. Fluorophores which were excited simultaneously by multiphoton excitation were detected in all tested cases.
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申请公布号 |
US6528802(B1) |
申请公布日期 |
2003.03.04 |
申请号 |
US20010806375 |
申请日期 |
2001.06.01 |
申请人 |
CARL ZEISS JENA GMBH |
发明人 |
KOENIG KARSTEN;HALBHUBER KARL-JUERGEN;FISCHER PETER;RIEMANN IRIS |
分类号 |
G01N21/64;C12Q1/68;G01N33/48;G01N33/50;G01N33/58;(IPC1-7):G01N27/26;G01J3/30 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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