Chimeric protein comprising a hypoxia inducible factor protein and a transcriptional activation domain and pharmaceutical use

摘要

Recombinant nucleic acid molecules encoding a chimeric transactivator protein are described. The chimeric transactivator protein includes a DNA binding domain of a DNA binding protein and a protein domain capable of transcriptional activation. Also provided are recombinant viral and non-viral vectors that are able to infect and/or transfect and sustain expression of a biologically active chimeric transactivor proteins in mammalian cells. Also provided are host cell lines and non-human transgenic animals capable of expressing biologically active chimeric transactivator proteins. A schematic of the adenoviral sequences contained within the HIF-lá/VP16 adenoviral vectors is shown. This vector is identical to the Ad2CMVBgal-6 backbone except that the B-gal gene has been replaced with the HIF-lá/VP16 hybrid construct.