发明名称 |
Isolation of membrane bound ligand-specific complexes |
摘要 |
A method to recover or "deracinate" receptors from intact membranes in their native configuration without removing microenvironmental components associated with them is described. The membranes containing the receptors are first associated with ligand-coupled solid supports to achieve association of the membranes through a receptor/ligand complex which includes the microenvironment to the solid support and then removing the membrane from the receptor and its microenvironment by shear forces. The thus isolated receptors and their microenvironments can then be analyzed for their role in cellular differentiation, apoptosis, transformation and the like.
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申请公布号 |
US2003040026(A1) |
申请公布日期 |
2003.02.27 |
申请号 |
US20020209328 |
申请日期 |
2002.07.29 |
申请人 |
JAKOBOVITS AYA;CHALLITA-EID PIA M. |
发明人 |
JAKOBOVITS AYA;CHALLITA-EID PIA M. |
分类号 |
G01N33/48;C07K14/705;G01N33/566;(IPC1-7):G01N33/567 |
主分类号 |
G01N33/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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