| 摘要 |
<p>A novel method of labelling cRNAs for probing oligo-based microarrays is disclosed. The polynucleotide template of interest is reverse transcribed into double-stranded cDNA using random primers that include an RNA polymerase promoter. This cDNA is then used as a template for synthesis of labeled RNA via in vitro transcription in the presence of labeled precursors.</p> |
