METHOD AND APPARATUS FOR RAPID DETERMINATION OF LIGAND-PROTEIN BINDING USING CHARCOAL ADSORPTION

摘要

A method for evaluating binding of a ligand to a target protein. The method includes the steps of: (a) providing a sample comprising a target protein an d a ligand, wherein the target protein and ligand are suspected to be bound reversibly together in a complex; (b) preconditioning activated charcoal wit h the target protein; (c) contacting the sample with the preconditioned activated charcoal for a time sufficient to allow for adsorption of unbound ligand to the activated charcoal; (d) eluting the sample from the activated charcoal; and (e) determining an amount of ligand in the eluted sample to thereby evaluate binding of the ligand to the target protein. An apparatus (Figure 1) useful in carrying out the method, and a method of making the sam e, are also disclosed.