发明名称 |
Cloned genes encoding reverse transcriptase lacking RNase H activity |
摘要 |
The invention relates to a gene which encodes reverse transcriptase having DNA polymerase activity and substantially no RNase H activity. The invention also relates to vectors containing the gene and hosts transformed with the vectors of the invention. The invention also relates to a method of producing reverse transcriptase having DNA polymerase activity and substantially no RNase H activity by expressing the reverse transcriptase genes of the present invention in a host. The invention also relates to a method of producing cDNA from mRNA using the reverse transcriptase of the invention. The invention also relates to a kit for the preparation of cDNA from mRNA comprising the reverse transcriptase of the invention.
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申请公布号 |
US2003039988(A1) |
申请公布日期 |
2003.02.27 |
申请号 |
US20010024149 |
申请日期 |
2001.12.21 |
申请人 |
KOTEWICZ MICHAEL LESLIE;GERARD GARY FLOYD |
发明人 |
KOTEWICZ MICHAEL LESLIE;GERARD GARY FLOYD |
分类号 |
C07H21/04;C07K14/16;C12N7/00;C12N7/01;C12N9/12;C12N9/22;C12N15/54;C12P19/34;C12Q1/68;C12Q1/70;(IPC1-7):C12Q1/70 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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