| 摘要 |
The present invention relates to methods for producing recombinant proteins, in particular recombinant secretory proteins, to a method for identifying nucleic acid molecules the expression products of which permit improved secretion of a recombinant secretory protein, to the use of molecules thus identified for enhancing secretion of heterologous proteins, and to corresponding kit systems. The method according to the invention makes provision for expression in a suitable host cell of a nucleic acid coding for a secretory protein together with a nucleic acid coding for a polypeptide having the biological activity of the eukaryotic translation initiation factor 4 E and/or for a polypeptide having the biological activity of a CaM kinase. It was found that coexpression of the translation initiation factor gene and/or of the CaM kinase gene leads to improved secretion of a desired recombinant protein from the cell.
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