MEASUREMENT OF MICROBIOLOGICAL ACTIVITY IN AN OPAQUE MEDIUM

摘要

A method for monitoring the microbiological contamination of opaque media is described and claimed. In this method, a Fluorogenic Dye is added to an Aliquot of opaque medium. After a certain time period, a fluorometer capable of measuring fluorescent signals in an opaque medium is used to measure the fluoregenic signals of the Fluorogenic Dye and the Reacted Fluorogenic Dye. A Useful RATIO of the fluorescent signal of the Reacted Fluorogenic Dye to the fluorescent signal of the Fluorogenic Dye is calculated and the information gleaned from the RATIO is used to ascertain the state of microbiological contamination in the opaque medium. Figure (1) shows a plot of fluorescent signals, in counts per second, of Resazurin and Resorufin in a Mineral Slurr y at Time Zero and at Time Future.