| 发明名称 |
A method for directional stable transformation of eukaryotic cells |
| 摘要 |
The invention provides DNA construct comprising in the 5' to 3' direction of transcription a promotor functional in a plant, an intron, a nucleotide sequence of interest, and a terminator region, said DNA construct comprising two non-identical recombination sites, where one of said non-identical recombination site is contained within said intron. |
| 申请公布号 |
AU757672(B2) |
申请公布日期 |
2003.02.27 |
| 申请号 |
AU19990015905 |
申请日期 |
1998.11.17 |
| 申请人 |
PIONEER HI-BRED INTERNATIONAL, INC. |
发明人 |
WILLIAM J. GORDON-KAMM;ALEXANDER LESZEK LYZNIK;CHRISTOPHER L. BASZCZYNSKI |
| 分类号 |
C12N9/00;C12N15/10;C12N15/113;C12N15/29;C12N15/31;C12N15/63;C12N15/82;C12N15/90;(IPC1-7):C12N15/82;A01H5/00;A01H5/10 |
主分类号 |
C12N9/00 |
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