METHOD OF OBTAINING OF ANTIOXIDANTS AND BIOACTIVE COMPOUNDS OF LIPIDIC NATURE

摘要

Method of successive obtaining of antioxidant and bioactive compounds of lipidic nature, in particular, beta-carotene, ergosterin, ubiquinones, where the following stages are implemented; a) extracting of Blakeslea trispora fungus with humidity of 7 - 75% with polar solvent (acetone for example) or with mixture of polar and nonpolar solvents (acetone and hexane, or acetone and ethanol for example) at temperature 40-70 degree C in 3 steps at biomass ratio: extraction equal 1:3.5 on the first step, 1:2 on the second step and 1:1.5 on the last step and the obtained extract is to be separated from bioschrotte by hot filtration at 50-60 degree C; b) Carotinoids (beta-carotene or lycopene) are to be crystallized, from the extract obtained on stage a), by mixture of polar and nonpolar solvents at 5 - 20 degree C during 3-24 hours, weak mixing and the settled carotinoids are separated by filtration and washed by ethanol; c) Phospholipins are precipitated from the remaining solution at the temperature of 5 - 20 degree C during 1 - 5 hours at weak mixing with the mixture of acetone nonpolar solvent, for example, hexane and obtained phospholipins are to be directed for the further fractional separation in the solution of ethanol with isolation of lecithin fraction. d) Remaining solution of neutral lipids is saponified in acetone medium with aqueous solution of potassium hydroxide at 50-70 degree C during 20-60 minutes and saponified fraction is to be diluted with water and hydrolyzed by sulphuric acid with obtaining of fatty acids mixture; e) Ergosterin in hexane is crystallized from nonsaponified fraction at 0 - 20 degree C during 4 hours with ratio hexane: nonsaponifiable fraction, equal 1:3 -1:5, and ergosterin obtained by crystallization process is filtrating, saponifying with organic solvent and drying; f) ubiquinones are recovered from the filtrate of nonsaponified fraction by elution from chromatographic column with the mixture polar and nonpolar solvents, in particular, hexane and acetone, for example, 1 - 5% solution of acetone in hexane and, g) ubiquinones are crystallized from eluted fraction with ethyl alcohol at -15 - +15 degree C, after the sediment of summary (Q-9,10) ubiquinones, formed during the crystallization process, is recovered and is to be refined if necessary.