发明名称 |
HIGH-THROUGHPUT SCREENING OF EXPRESSED DNA LIBRARIES IN FILAMENTOUS FUNGI |
摘要 |
The invention provides a method for the expression of exogenous DNA libraries in filamentous fungi. The fungi are capable of processing intron-containing eukaryotic genes, and also can carry out post-translational processing steps such as glycosylation and protein folding. The invention provides for the use of fungi with altered morphology, which permits high-throughput screening and directed molecular evolution of expressed proteins. The same transformed fungi may be used (as shown in figure 1) to produce larger quantities of protein for isolation, characterization, and application testing, and may be suitable for commercial production of the protein as well.
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申请公布号 |
WO0179558(A8) |
申请公布日期 |
2003.02.27 |
申请号 |
WO2001US12335 |
申请日期 |
2001.04.13 |
申请人 |
EMALFARB, MARK, AARON |
发明人 |
EMALFARB, MARK, AARON;PUNT, PETER, JAN;VAN ZEIJL, CORNELIA;VAN DEN HONDEL, CORNELIUS |
分类号 |
C12N15/09;C12N1/00;C12N15/10;C12P21/02;C12Q1/04;C12R1/645;(IPC1-7):C12Q1/68;C12N1/14;C12N15/00;C07H21/02 |
主分类号 |
C12N15/09 |
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