| 摘要 |
<p>The present invention concerns methods for culturing not immediately culturable (NIC) bacteria in a sample, comprising the steps of: a) contacting said mixture containing said NIC bacteria with a bacterial growth autoinducer, said bacterial growth autoinducer being characterised in that it has the following properties: i) it can be produced by a Gram-negative bacterium in response to noradrenaline in serum SAPI medium; ii) it is stable for 45 minutes at 100°C; iii) it is stable to lyophilisation; iv) it has a negative charge and binds to an anion exchange resin; v) it is polar and hydrophilic, is soluble in water, and substantially insoluble in methanol, propanol, butanol, n-hexane, CHCl3 and DMSO; vi) it has a molecular weight of approximately 1000 Daltons; and b) culturing said sample. Also provided are diagnostic test methods for same.</p> |
