| 摘要 |
PROBLEM TO BE SOLVED: To accurately calculate the maximum reaction speed Vmax and a Michaelis constant Km. SOLUTION: This method for analyzing an enzymatic reaction contains a step for calculating such a Km as satisfying the following equation:Σ(vi/ si)[1/(1+Km/si)]/Σ(1/si)[1/(1+Km/si)<2> ]-Σ(vi/si<2> )[1/(1+Km/si)<2> ]/Σ(1/si<2> )[1/(1+ Km/si)<3> ]=0, and a step for applying the Km to the following equation: Vmax=Σ(vi/si)[1/(1+Km/si]/Σ(1/si)[1/(1+Km/si)<2> ] to calculate the Vmax, when a substrate concentration si as a measurement result for the change of an absorbance at a constant wavelength in a sample solution having an enzymatic activity and the reaction speed vi[(i)=1 to (n)] of the enzymatic reaction are given.
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