发明名称 |
High efficiency methods for combined immunocytochemistry and in-situ hybridization |
摘要 |
The invention provides a high efficiency method for combined immunocytochemistry and in situ hybridization. In one aspect, the method is used to simultaneously determining a cell phenotype and genotype by contacting a cell with an antigen-specific antibody bound to a ligand, contacting the cell with polynucleotide probe to form a complex of the probe and a nucleic acid in the cell, contacting the cell with a detectably labeled anti-ligand, and detecting the polynucleotide-probe complex and the anti-ligand-ligand complex. The presence of the anti-ligand is correlated with the presence of the antigen and the presence of the probe-nucleic acid complex is correlated with the presence of the nucleic acid in the cell.
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申请公布号 |
US6524798(B1) |
申请公布日期 |
2003.02.25 |
申请号 |
US20000592120 |
申请日期 |
2000.06.12 |
申请人 |
APPLIED IMAGING CORPORATION |
发明人 |
GOLDBARD SIMON;HONG TSAI-HSIA;ZOCCOLI MICHAEL A.;LIN EMILY |
分类号 |
C12Q1/68;G01N33/569;G01N33/574;(IPC1-7):G01N33/53;G01N33/542;C07H21/04;C07H21/02 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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