PURIFIED NUCLEIC ACID ENCODING A THERMOSTABLE PYROPHOSPHATASE

摘要

A DNA that encodes a thermostable pyrophosphatase (PPase) is provided. The DNA is obtained using a DNA probe consisting of SEQ ID NO: 1. Also provided are vectors comprising said DNA and host cells transformed with suc h a vector. The invention also provides methods for producing thermostable pyrophosphatase, and methods for isolating a target DNA fragment comprising a DNA coding for a thermostable pyrophosphatase from a bacterium of the genus Thermus. The thermostable pyrophosphatase prepared in accordance with the pr esent invention is preferably packed in a kit and can be used in a nucleic acid am plification method.